Direct PCR Kits & Master Mixes

Direct PCR Kits & Master Mixes contain all the reagents needed for quick amplification of genomic DNA from crude samples. The kits / master mixes can be also applied to direct amplification of exogenous target genes, such as virus, bacteria and parasites from blood. The kits / master mixes are optimized with our unique inhibitor-resistant DNA polymerases and buffers for best performance. There is the choice of an inert green dye in the mixes, used for direct gel loading. The PCR products can be directly loaded onto agarose gel without addition of loading dye for electrophoresis.

    GC version works for GC-rich and complex target.

    Highlight

    • Time saving: No need for DNA extraction or pre-lysis of samples as other similar commercial kits / master mixes
    • Convenient and flexibility:  For the kitsonly need adding enzyme, primers and samples in 2X mix. The enzymes are packed in separated vials. The customers are able to titrate the amount of enzymes to find the best one for their targets. For the master mixes, the enzymes are included inside whole master mixes, so you only need adding primers and samples in 2X Master Mix
    • High inhibitor-resistance: Can amplify targets in PCR containing high concentration of PCR inhibitors 
    • Specificity: GC version works for >65% GC content and complex target, increases specificity
    • Simply analysis: Directly loading products for electrophoresis after PCR

    Applications

    • Genotyping
    • Mutation detection
    • Transgene detection
    • Knockout analysis
    • Sequencing
    • Pathogen detection
    • Forensic identification
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