Standard PCR Kits

Standard PCR Kits are 2X concentrated solutions of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. The pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mixes are optimized for efficient and reproducible PCR to amplify the target gene from purified DNA.

The Master Mix has four versions, with standard buffer and GC buffer, colorless and green. The Master Mix with GC buffer is used for amplification of GC-rich target (GC content is >65%). The green one is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

On a 1% agarose gel in 1X TBE, one dye migrates at approximately 4 kb and another migrates at approximately 50 bp.

Highlights

• Convenient: Ready-to-use mix, direct loading of PCR products on gel
• Thermostable: Enzyme in the mix retains at least 85% activity after 60 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotide)
• Efficient amplification of complex target