2X ReadyGo HSF PCR Master Mix

2X ReadyGo HSF PCR Master Mix is a 2X concentrated solution of hot-start and high fidelity Taq DNA Polymerases, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. It increases specificity and sensitivity because reducing formation of no specific products and primer dimer. You can set up reactions at room temperature. The mix is optimized for efficient and reproducible PCR.

The Master Mix has four versions, with standard buffer and GC buffer, colorless and Green. The Master Mix with GC buffer is used for amplification of GC-rich target (GC content is >65%). The green one is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Highlights

• Convenient: Ready-to-use mix, direct loading of PCR products on gel
• Thermostable: Retain at least 85% activity after 60 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotide)

Data Sheet: 2X ReadyGo HSF PCR Master Mix