KlenTaq is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability. It has some inhibition-resistant features even it is not as strong as our new generation of Klentaq or Taq mutants. This enzyme can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, however, it can not be used in real-time PCR TaqMan assay which requires 5’-exonuclease activity.
KlenTaq LA version (Long & Accurate) is available, which can be used to amplify a longer gene target with lower error rate.
KlenTaq and KlenTaq LA Mix are available as a ready-to-use 2X mixture of DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction and there is the choice of an inert green dye, used for direct gel loading. All you have to do is to add template, specific primers and water (Click here).