Suprem TaqTM is a new generation mutant of Taq polymerase. The mutations in Taq polymerase gene render the enzyme resistant to the inhibitory effects of higher concentrations of blood, soil, plant and more. It typically remains functional in 40% whole blood in PCR, and in some concentrations of crude soil extracts where other commercial enzymes fail. It can tolerate more in the presence of PCR enhancer cocktail. It is able to amplify the target gene directly from whole blood, serum, plasma, water, milk, tissue and plant leaf, etc. without DNA purification prior to PCR. This enzyme can be used in real-time PCR with both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assay which requires 5’-3’ exonuclease activity.
Suprem TaqTM LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy.
Suprem TaqTM and Suprem TaqTM LA Mix are available as a ready-to-use 2X mixture of DNA polymerase, salts, magnesium and dNTPs for setting up a trouble-free PCR reaction and there is the choice of an inert green dye, used for direct gel loading. All you have to do is to add template, specific primers and water (Click here).