I used to have a problem to amplify a target from DNA purified with DNA extraction kit. I tested a lot of Taq enzymes available in the market and eventually found your Taq mutant enzymes worked best.
When I visited your website I didn't believe that Direct RT-PCR Kit was able to directly amplify RNA target from whole blood and serum without RNA extraction. It's amazing after I tested it. It worked like a charm!
It's difficult to recover DNA from ancient smelt vertebrae. I combined Taq mutant enzyme with PCR enhancer in the PCR according to your suggestion. It worked great.
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