I used to have a problem to amplify a target from DNA purifed with DNA extraction kit. I tested a lot of Taq emzymes availabe in the market and eventually find your Taq mutant enzymes work best.
When I visited your website I didn't believe that Direct RT-PCR Kit was able to directly amplified RNA target from whole blood without RNA extraction. It's amzing after I tested it. It works as a cham!!
It's difficult to recover DNA from acient smelt vetebrae. I combined Taq mutat enzyme with PCR enhancer in the PCR according to your suggestion. It works great.
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