In an effort to overcome PCR inhibition, boost PCR amplification, and simplify the PCR protocol, we have developed PCR enhancer cocktails (PECs). These cocktails, in combination with inhibitor-resistant Taq mutant enzymes, enable efficient amplification of exogenous, endogenous, and high GC-rich DNA targets directly from crude samples, including human plasma, serum, whole blood, some food samples, and plant tissue, without the need for DNA purification.
In the presence of these enhancer cocktails, the Taq mutant enzyme can tolerate up to 40% whole blood, plasma, and serum, as well as templates with up to 80% GC content in PCR. Furthermore, these enhancer cocktails improve the performance of the novel Taq mutants in real-time PCR amplification using crude samples, in both SYBR Green fluorescence detection and TaqMan assays.
Our PEC Combo contains a combination of five PECs to allow you to test which one works best for your application.