The Core Direct qPCR Master Mixes are designed for rapid quantitative analysis of target DNA directly from various sample types, including blood, serum/plasma, swabs, cell cultures, bacterial colonies, and animal or plant tissue. These master mixes enable robust amplification without requiring prior DNA purification procedures.
The Core Direct qPCR Master Mixes utilize a unique inhibitor-resistant DNA polymerases and buffer system that is optimized for optimal performance. In most cases, samples can be added directly to the PCR master mix without the need for pre-lysis.
In the master mixes, the enzymes are included in the 2X master mixes for convenience. The master mixes ensure fast and easy preparation with a minimum of pipetting steps and are especially recommended for rapid and easy preparation of high-quality qPCR reactions.
Direct detection of viral or bacterial DNA in nasal or throat swabs
Direct qPCR from blood samples and other body fluid
Direct qPCR from cell culture
Direct colony screening or quantitative analysis of target DNA from single colony
Direct amplification of target DNA from various tissue samples