Reverse Transcriptases

Reverse Transcriptases are mutational derivatives of Moloney-Murine Leukemia Virus Reverse Transcriptase. They are specifically designed to reverse transcribe low-abundance or degraded RNA, and it offers significantly better resistance to contaminants and inhibitors such as reagents used during RNA extraction and contaminants from biological samples.

The enzymes are highly processive and sensitive, which allows for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors. They are the engineered reverse transcriptases that can offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 50-75ºC. Therefore, they are the enzymes of choice for both daily or demanding RNA reverse transcription applications.

Highlights

  • Thermostable from 50-75°C
  • Transcribes degraded samples and resists inhibitors / contaminants
  • Provides high cDNA yields from difficult or ultra-low RNA samples
  • High yields of full-length cDNA up to 15-20 kb
  • Efficient—complete cDNA synthesis in 15 to 30 minutes