The Core Direct qRT-PCR Kits are designed for real-time quantitative analysis of target RNA directly from blood, serum/plasma, swabs, cell cultures, bacterial colonies, and animal or plant tissue. The kit enables robust amplification without requiring prior RNA purification procedures.
The kits are optimized with our unique inhibitor-resistant, thermostable polymerases and buffer system for optimal performance. In most cases, samples can be added directly to the RT-PCR master mix without the need for pre-lysis.
The blend of polymerases are packaged separately from the 2X RT-PCR Mix, allowing scientists to optimize the enzyme amount for their specific application based on the inhibitory level in the samples. The kits ensures fast and easy preparation with a minimum of pipetting steps and are especially recommended for:
Direct detection of viral or bacterial RNA in nasal or throat swabs
Direct qRT-PCR from blood samples and other body fluid
Direct qRT-PCR from cell culture
Direct quantitative analysis of target RNA from single colonies or cells
Direct amplification of target RNA from various tissue samples
Unique Invention:First One-Step Direct qRT-PCR systems in the market that are able to tolerate high concentration of PCR inhibitors
Time saving: No need for RNA extraction, one-step, performance of RT and PCR in same reaction
Convenient: Only need adding enzymes, primers, probe / DNA binding dye and sample in 2X mix
High inhibitor-resistance: Can amplify RNA target in reaction containing high PCR inhibitors.