The Core Direct RT-PCR Kit is designed to analyze target RNA directly from blood, serum/plasma, swabs, cell cultures, bacterial colonies, and animal or plant tissue. The kit enables robust amplification without requiring prior RNA purification procedures.
The kit is optimized with our unique inhibitor-resistant, thermostable polymerases and buffer system for optimal performance. In most cases, samples can be added directly to the RT-PCR master mix without the need for pre-lysis.
The blend of polymerases is packaged separately from the 2X RT-PCR Mix, allowing scientists to optimize the enzyme amount for their specific application based on the inhibitory level in the samples. The kit ensures fast and easy preparation with a minimum of pipetting steps and is especially recommended for:
Direct detection of viral or bacterial RNA in nasal or throat swabs
Direct amplification from blood samples and other body fluid
Direct PCR from cell culture
Direct detect RNA from colony or cells
Direct amplification of target RNA from various tissue samples
Unique Invention:First One-Step Direct RT-PCR systems in the market that is able to tolerate high concentration of PCR inhibitors
Time saving: No need for RNA extraction, one-step, performance of RT and PCR in same reaction
Convenient: Only need adding enzymes, primers and sample in 2X mix
High inhibitor-resistance: Can amplify RNA target in reaction containing high PCR inhibitors