This buffer is formulated for use in PCR with full-length Taq mutant DNA polymerases and corresponding LA versions.
Each tube of 1.5 ml of 10X PCR reaction buffer is enough for 600 x 25 µl reactions. The 10X buffer composition is 500 mM Tris-HCl pH 9.1, 160 mM ammonium sulfate, 0.25% Brij® 58, and 25 mM magnesium chloride.
Data Sheet: 10X Taq Mutant PCR Buffer