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Hot-start KlenTaq DNA Polymerase is a mixture of KlenTaq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature, preventing primer dimer and spurious amplification to increase specificity.
This enzyme is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability. It can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, however, it not be used in real-time PCR TaqMan assay, which requires 5’-exonuclease activity.
Data Sheet: Hot-start KlenTaq