Hot-start KlenTaq DNA Polymerase is a mixture of KlenTaq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures. This prevents primer-dimer formation and spurious amplification, increasing specificity. During normal cycling conditions, the inhibitor releases the enzyme, allowing efficient amplification.
KlenTaq DNA Polymerase is a Taq polymerase variant that lacks 5’-exonuclease activity due to an N-terminal deletion of Taq. This modification results in improved fidelity and thermostability compared to the wild-type Taq. Hot-start KlenTaq DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it cannot be used in real-time PCR TaqMan assays, which require 5’-exonuclease activity.
Data Sheet: Hot-start KlenTaq