Hot-start OmniTaq 3 DNA Polymerase is a mixture of OmniTaq 3 DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature, preventing primer dimer and spurious amplification to increase specificity.
This enzyme is a new generation mutant of Taq DNA polymerase that can tolerate high level of PCR inhibitors. It can be used in real-time PCR both with DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assay that requires 5’-exonuclease activity.
Data Sheet: Hot-start OmniTaq 3