Hot-start ZipTaq DNA Polymerase is a novel enzyme mixture composed of ZipTaq DNA Polymerase and an aptamer-based inhibitor. This inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at low temperatures to prevent non-specific amplification such as primer dimer formation, but releases the enzyme during normal cycling conditions to enable efficient amplification. The hot-start feature of this enzyme allows reactions to be set up at room temperature, increasing specificity and sensitivity of PCR.
This enzyme is a new generation mutant of Taq polymerase with multiple mutations that make it the fastest enzyme in our collection. With the addition of one of our PECs, it can tolerate up to 40% blood in the reaction. This enzyme can be used in real-time PCR with both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assay which requires 5’-3’ exonuclease activity.
Data Sheet: Hot-start ZipTaq