KlenTaq-S & KlenTaq-S LA

KlenTaq-S DNA Polymerase is a 5’-exonuclease deficient Taq polymerase (an N-terminal deletion of Taq) with improved fidelity and thermostability, as well as an extra mutation providing the enzyme with PAP function (Pyrophosphorolysis-activated polymerization). The enzyme is capable of detecting rare mutations, large heterozygous deletions, gene duplications, and more, even in the presence of a large excess of wild-type allele and inorganic pyrophosphate. In theory, PAP can detect a single base mutation in 3x10^11 copies of wild-type allele.

This enzyme can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assay, which requires 5’-exonuclease activity. If a TaqMan assay is required, it can be blended with our full-length Taq mutant enzymes.

KlenTaq-S LA DNA Polymerase is a Long and Accurate version of KlenTaq-S, which is a Taq polymerase with an N-terminal deletion that makes it 5’-exonuclease deficient. KlenTaq-S LA DNA Polymerase has enhanced fidelity and thermostability, as well as an additional mutation that enables it to perform pyrophosphorolysis-activated polymerization (PAP) function. These properties make it suitable for amplifying longer gene targets and detecting rare mutations, large heterozygous deletions, and gene duplications even in the presence of a large excess of wild-type alleles and inorganic pyrophosphate.

KlenTaq-S LA DNA Polymerase can be used in real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green, but it cannot be used in real-time PCR TaqMan assays that require 5’-exonuclease activity. However, it can be blended with full-length Taq mutant enzymes for TaqMan assays.

Application:

1. Rare mutations detection
2. Large heterozygous deletions

Data Sheet: KlenTaq-S, KlenTaq-S LA