OmniTaq 3 & OmniTaq 3 LA

OmniTaq 3 DNA Polymerase is a new generation mutant of Taq polymerase that is resistant to inhibitory effects from higher concentrations of blood, soil, plants, and more. It remains functional in 40% whole blood in PCR, even without PCR enhancer, and in some concentrations of crude soil extracts where other commercial enzymes fail. However, the presence of a PCR enhancer cocktail can further improve its tolerance. It can directly amplify the target gene from various sample types without DNA purification prior to PCR. This enzyme can be used in real-time PCR with both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assay which requires 5’-3’ exonuclease activity.

OmniTaq 3 LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy. It is compatible with real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it is recommended to use the non-LA version for TaqMan assays in real-time PCR.

• Thermostability – more thermostable than standard Taq DNA polymerase
• Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product
• Robust and Inhibition-resistant – reliable direct amplification from crude samples without DNA purification and with even the most challenging DNA targets, especially when combining with our powerful PCR enhancers
• Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples

Data Sheet: OmniTaq 3, OmniTaq 3 LA