OmniTaq & OmniTaq LA

OmniTaq DNA Polymerase is a mutant of Taq polymerase that confers resistance to the inhibitory effects of blood, soil, and other contaminants. It has been shown to remain functional in up to 20% whole blood, particularly when used with one of our PCR enhancer cocktails, as well as in some concentrations of crude soil extracts where other commercial enzymes have failed. This enzyme is compatible with real-time PCR using both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assays that require 5'-exonuclease activity.

OmniTaq LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy. This enzyme is compatible with real-time PCR using DNA binding dyes like SYBR Green and Eva Green. However, for real-time PCR TaqMan assays, it is better to use the non-LA version.

• Thermostability – more thermostable than standard Taq DNA polymerase
• Efficient – novel buffer system maximizes efficiency of PCR amplification, increases yield of any PCR product
• Robust and Inhibition resistant – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets, especially when combining with our PCR enhancers
• Flexible – LA version is ideal for amplifying any target up to 30 kb from DNA extracted from human, animal and plant samples
• Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions even for crude samples

Data Sheet: OmniTaq, OmniTaq LA