ZipTaq & ZipTaq LA

ZipTaq DNA Polymerase is a new generation mutant of Taq polymerase with multiple mutations that make it the fastest enzyme in our collection. With the addition of one of our PECs, it can tolerate up to 40% blood in the reaction. This enzyme can be used in real-time PCR with both DNA binding dyes, such as SYBR Green and Eva Green, and TaqMan assay which requires 5’-3’ exonuclease activity.

ZipTaq LA version (Long & Accurate) is available, which allows for amplification of longer products with higher fidelity and accuracy. It is compatible with real-time PCR with DNA binding dyes, such as SYBR Green and Eva Green. However, it is recommended to use the non-LA version for TaqMan assays in real-time PCR.

• Thermostability – more thermostable than standard Taq DNA polymerase
• The fasted enzyme – mutations of enzyme and novel buffer system enable amplification of 1kb target with one second annealing / extension time.
• Robust and Inhibition-resistant – reliable direct amplification from crude samples without DNA purification when combining with our powerful PCR enhancers.

Data Sheet: ZipTaq, ZipTaq LA